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Theme #3. The fundamentals of genetic engineering

2. The aim: to form at students ideas of the basic principles and methods of genetic engineering technology and their application in medicine.

3. The tasks:

3.1. Allocation of the set gene DNA from a genome.

3.2. Transfer of genes into the cells of other organisms.

3.3. Transgene plants.

3.4. DNA diagnostics.

3.4. Gene therapy of hereditary diseases.

4. Performance form: glossary compiling.

4.1. Alternative splicing - _____________________________________

___________________________________________________________

4.2. Amplification - _________________________________________

___________________________________________________________

4.3. Antigen - _______________________________________________

___________________________________________________________

4.4. Autoradiography - ______________________________________

___________________________________________________________

4.5. Bacteriophage - ___________________________________________

___________________________________________________________

4.6. Blotting - ______­­­­­­­­­­­___________________________________________

___________________________________________________________

4.7. Vector - ________________________________________________

___________________________________________________________

4.8. Genetic code - ___________________________________________

___________________________________________________________

4.9. Genetic engineering - _____________________________________

___________________________________________________________

4.10. Gene - __________________________________________________

___________________________________________________________

4.11. Genome - ________________________________________________

___________________________________________________________

4.12. Gene bibliotheque - _______________________________________

___________________________________________________________

4.13. Gene therapy - ­_­­­­­­­­­­­­­­________________________________________

___________________________________________________________

4.14. Hybridome - ___________________________________________

_____________________________________________________________

4.15. DNA polymerase - ______________________________________

___________________________________________________________

4.16. Intron - _______________________________________________

___________________________________________________________

4.17. DNA cloning - ­­­­­­­­­­­­­­­­­­­­­­­­­­__________________________________________

__________________________________________________________

4.18. Mapping - _____________________________________________

___________________________________________________________

4.19. Complementary DNA - ____________________________________

___________________________________________________________

4.20. Cosmide - ______________________________________________

___________________________________________________________

4.21. Genetic code - _________________________________________

___________________________________________________________

4.22. Codon (triplet) - ________________________________________

___________________________________________________________

4.23. Ligase - ______________________________________________

____________________________________________________________

4.24. “Cohesive” ends - ________________________________________

____________________________________________________________

4.25. Complementarity - ______________________________________

____________________________________________________________

4.26. Locus - ________________________________________________

____________________________________________________________

4.27. Methylation - __________________________________________

____________________________________________________________

4.28. DNA marker - ___________________________________________

____________________________________________________________

4.29. Modification - __________________________________________

____________________________________________________________

4.30. Mutagen - ______________________________________________

____________________________________________________________

4.31. Mutation - ______________________________________________

____________________________________________________________

4.32. Cistron - ______________________________________________

____________________________________________________________

4.33. Nucleotide - _____________________________________________

_____________________________________________________________

4.34. Northern blotting - ________________________________________

____________________________________________________________

4.35. Gyrase - _______________________________________________

____________________________________________________________

4.36. Polymorphism - __________________________________________

____________________________________________________________

4.37. Promoter - ______________________________________________

_____________________________________________________________

4.38. Reverse transcriptase - ____________________________________

____________________________________________________________

4.39. Restriction - ____________________________________________

____________________________________________________________

4.40. Recombinant DNA - _____________________________________

____________________________________________________________

4.41. Plasmid - _____________________________________________

____________________________________________________________

4.42. PCR - __________________________________________________

____________________________________________________________

4.43. Screening - _____________________________________________

______________________________________________________________

4.44. Splicing - ______________________________________________

____________________________________________________________

4.45. Restriction site - _______________________________________

______________________________________________________________

4.46. Southern blotting - ______________________________________

____________________________________________________________

4.47. Transgenic organism - ___________________________________

____________________________________________________________

4.48. Transduction - __________________________________________

____________________________________________________________

4.49. Transformation - ________________________________________

____________________________________________________________

4.50. Selective medium - ____________________________________

____________________________________________________________

4.51. Phasmid - _______________________________________________

____________________________________________________________

4.52. Chromatin - _____________________________________________

____________________________________________________________

4.53. Chromatids - ____________________________________________

____________________________________________________________

4.54. Chromosomes - ___________________________________________

____________________________________________________________

4.55. Exon – ________________________________________________

____________________________________________________________

4.56. Gene expression - _______________________________________

____________________________________________________________

4.57. Electrophoresis - _________________________________________

____________________________________________________________

4.58. Nuclear genetics - ______________________________________

____________________________________________________________

4.59. Endonuclease - _________________________________________

____________________________________________________________

4.60. Exonuclease - __________________________________________

____________________________________________________________

5. Performance criteria:

а) studying of basic literary sources on the researched problem;

b) information processing;

c) glossary compiling and design;

Requirements to glossary design:

а) the volume of glossary should include not less than 50 terms and definitions;

b) definitions should be complete and brief, without extra explanations.

6. Deadline: before the midterm control on the chapter “Molecular cytobiology”.

7. Criteria of assessment: - 100 points at maximum that corresponds to 100%

8. Bibliography:

8.1. Албертс Б. и др. Молекулярная биология клетки. М., 1994.

8.2. Алиханян С.И., Акифьев А.П. Общая генетика. М., 1985.

8.3. Гинтер Е.К. Медицинская генетика. М., 2003.

8.4. Глеба Ю.Ю. Биотехнология растений. СОРОСовский образовательный журнал, №6, 1998.

8.5. Жимулев И.Ф. Общая и молекулярная генетика. Новосибирск, 2006.

8.6. Лещинская И.Б. Генетическая инженерия. №1, 1996.

8.7. Льюин Б. Гены. М., 1987.

8.8. Интернет сайты:

http://www. nih.gov

http://genlink.wustl. edu

http://www. geneletter. org

http://www. wiley. co. uk/genetherapy

http://www. genom. gov.

9. Check:

9.1. Restrictase properties and their role in genes transfection.

9.2. Main stages of genes cloning.

9.3. Genetic maps making up and their significance.

9.4. Methods of recombinant DNA obtaining.

9.5. Modern achievements of genetic engineering in medicine.

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