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Figure 2

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Characterization of interleukin-17 (IL-17)–positive T cells in patients with juvenile idiopathic arthritis (JIA). Peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) from JIA patients were analyzed by flow cytometry. A and B, Representative dot plots of paired PBMCs and SFMCs stained for CD3, IL-17, and T cell receptor γ/δ (TCRγ/δ) (A) as well as for CD3, IL-17, and CD8 (B), both gated on live lymphocytes and CD3+ cells. C, Representative dot plot of JIA SFMCs stained for CD3, CD4, CD45RO, and IL-17 gated on live lymphocytes, CD3+ cells, and CD4+ cells. Numbers in each compartment are the percentage of cells.

 

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