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Figure 5

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Dysregulated cytokine expression by interleukin-17 (IL-17)–positive, CD4+ T cells in the synovial fluid of juvenile idiopathic arthritis (JIA) patients. A, Representative dot plots of paired samples of peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) from patients with JIA. Cells were stained for intracellular cytokines IL-17, interferon-γ (IFNγ), IL-22, and IL-4 and analyzed by flow cytometry, gated on live lymphocytes and CD3+,CD4+ cells. Numbers in each compartment are the percentage of cells. B, Immunohistologic localization of IL-17 and IL-22 in JIA synovial tissue. Frozen sections of synovial biopsy tissue were stained for CD4, IL-17, and IL-22 by standard immunohistochemical techniques. Tissue sections show typical hypertrophied synovium and dense inflammatory infiltrate. Positive cells expressing the marker of interest are stained brown; arrow shows typical positive cells. Bar = 20 μm.


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