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Figure 1

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Enrichment of interleukin-17 (IL-17)–positive, CD4+ T cells in synovial fluid from patients with juvenile idiopathic arthritis (JIA) and correlation with clinical subtype. A, Representative dot plots of paired samples of peripheral blood mononuclear cells (PBMCs) and synovial fluid mononuclear cells (SFMCs) from a patient with JIA. Cells were stained for surface expression of CD3 and CD4 and then for intracellular expression of IL-17 and were analyzed by flow cytometry, gated on live lymphocytes and CD3+ cells. Numbers in each compartment are the percentage of cells. B, Numbers of IL-17+ cells as a percentage of CD4+ T cells in SFMCs from JIA patients (n = 28), PBMCs from JIA patients (n = 22), and PBMCs from healthy controls (n = 9). Bars show the median. C, Numbers of IL-17+ cells as a percentage of CD4+ T cells in PBMCs (PB) and SFMCs (SF) from patients with persistent (n = 9 PBMC samples; n = 12 SFMC samples) and extended (n = 6 PBMC samples; n = 13 SFMC samples) oligoarticular JIA (O-JIA) and in PBMCs from healthy controls (n = 9). Bars show the median.

 


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