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Figure 6

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Preferential migration of interleukin-17 (IL-17)–positive, CD4+ T cells to the CCR6 ligand CCL20 as compared with CD4+ T cells. Purified CD4+ T cells from patients with juvenile idiopathic arthritis (JIA) and healthy controls were migrated to 10 ng/ml, 100 ng/ml, or 1,000 ng/ml of CCL20 or RPMI 1640–0.5% bovine serum albumin (BSA). A, Cells from healthy controls analyzed by intracellular cytokine staining for IL-17 and interferon-γ (IFNγ) before (left) and after migration to control medium (RPMI 1640–0.5% BSA) (middle) or 1,000 ng/ml of CCL20 (right). Cells were analyzed by flow cytometry, gated on live CD3+,CD4+ cells. Numbers in each compartment are the percentage of cells. Results are representative of 1 experiment of 5 experiments performed. B, Response of IL-17+,CD4+ T cells and all CD4+ T cells to a titration of the CCL20 gradient. Data are expressed as the chemotactic index (ratio of cells migrating to CCL20 divided by cells migrating to control medium). Values are the mean ± SEM of 5 experiments.

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